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KMID : 0613820050150040561
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Journal of Life Science
2005 Volume.15 No. 4 p.561 ~ p.565
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The Biochemical Properties of Korean radish (Raphanus sativus L.) and Its Seed Protein as a Natural Preservative : The Influences on Antigenotoxicity and Cytotoxicity
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Choi Yoon-Hyeok
Hwang Cher-Won
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Abstract
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In this study, we report antigenotoxicity and cytotoxicity of Korean Radish extract (RJ) and its seed protein (RSP) to non-tumoral 3T3 cell line. In the case of antigenotoxicity, each cell line was treated with 10 ㎕ of 100 ㎍/ml N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) before adding 10 ㎕ of 10 ㎎/ml RJ and 1 ㎎/ml RSP to the cell. Both RJ and RS were shown 30% and 43% of antigenotoxicity respectively. As a result of quantitative analysis for lactose dehydrogenase (LDH), no cytotoxic activity against 3T3 cells was detected when the cells were treated with various concentrations of RJ and RSP. RSP showed 85% of antimicrobial activity against cosmetic sample (C1) assumed as contaminated by bacteria. RSP and RJ showed 79% and 76% of antimicrobial activities repectively on another cosmetic sample (C4, contaminated by fungi) were treated with 10 ㎎/ml RJ and 1 ㎎/ml RSP
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